TY. BSc. Biotechnology

Semester VI: Paper-10: Theory

Unit III

       ·     History & future prospects of human genome projects

       ·     Microarray principle & applications

       ·     Overview of bioinformatics & applications

       ·     Introduction, need & types of databases

       ·     Biological databases: Nature of biological data, Importance

               of Biological Databases in Biological Discovery

      Study Materials 

Unit IV

        ·    Introduction to bibliographic databases: PubMed

        ·    Major bioinformatics resources: NCBI,EBI & ExPASy

        ·    Nucleic acid sequence databases: GenBank, EMBL,DDBJ

        ·    Protein sequence database: SWISS-PROT, TrEMBL

        ·    Homology and similarity search tools: BLAST, FASTA

Study Materials  

Unit V

         ·    Protein structure databases: PDB

         ·    Structural classification of Proteins

         ·    Multiple sequence alignment

         ·    Overview of comparative & functional genomics

         ·    Phylogenetic analysis

Study Materials  

Semester VI: Paper-10: Practical

1. Getting the gene sequences by exploring and queering the nuclic acid databases, GenBank, EMBL & DDBJ          

2. Getting the amino acid sequence by exploring and querying the protein sequence database. Swiss Prot, Uniprot           

3. Introduction to literature database at NCBI and querying the PUBMED database using the ENTREZ search engine. 

4. Overview of OMIM & data retrieval   

5. Find the Databases similarity search through BLAST  

6. Multiple alignment of the give sequence by using ClustalW 

7. 3-D Protein structure visualization and measurement of bond length, bond angel, and torsion angels using Rasmol 

Figures for Practical 1,2 & 3  

First Year (Sem -II)

Theory: Unit IV:  Biocomputing

4.5

Practical:

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Ex:9a

Ex:9b

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Ex:12

(Note: "Right Click" on the above links and "save as" in your local Drive)